The Effects of Phytase

l   Reducing the amount of phosphor source in feedstuff such as bone powder, calcium hydrogen phosphate, lower the cost of feed formula

l   Increasing the utilization rate of mineral element, reducing the metabolic disease caused by the lack of mineral element；

l   Enhancing the utilization rate of nourishment materials such as protein, starch and so on，improving the growing performance of animals；

l   Broadening the space of feed formula, and improving the formula；

l   Eplacing the majority part of calcium hydrogenphosphate and bone powder, reducing the heavy metal poisoning and microbial contamination in the feed；

l   Decreasing the mill dust pollution during the feed processing，decreasing the waste of inorganic phosphorus material；

l   Decreasing the total phosphorus level in feed, reducing the phosphorus in excretion，consequently protecting the environment.

The Action Mechanism of Phytase

n Efficiently hydrolyzes phytate phosphorus which abundantly exists in feed, increasing the utilization rate of phosphorus in vegetal feed materials.

n Destroys the chelated structure of phytate, releases the chelated nutritive material such as mineral materials, protein, and starch; and enhances the utilization rate of nutritive components in feed material.

n Reduces the restrain of phytate to circadian activity substance in alimentary tract.

The Influence of Feed Processing Techniques to Phytase Activity

As a kind of protein, phytase is very easily influenced by environment factors. At present , the feed mills are using more and more heat processing equipments，such as expanding machine、extruding conditioner or other types of stress conditioner and high temperature shot time processing equipments. Those equipments will also destroy the thermal sensitivity components like enzyme while curing the feed. So the loss of phytase in feed heating is the most concerned problem in feed industry.

The normal temperature of feed pelleting is around 70-90℃expanding temperature can reach 120℃ upwards. People worry about that the special space structure of enzyme protein will be destroyed in such a high temperature, and lose its catalyses activity. Pelleting and expanding process also cause very high pressure, influenced by which, the volume, structure and activity part of enzyme protein may also change, leading to slower catalysis reacting speed, then shows the decrease of enzyme activity. The enzyme added in feed before pelleting would go through the processes of agitation, mixture, librations, extrusion, all of which will produce shearing force to act on the enzyme. Generally speaking, enzyme activity will decrease with the increase of shearing force and the process of time. And, the moisture content in feed will be increased during the process of pelleting, enzyme protein will change due to change of surface tension. And the existing free water will also destroy the interaction of hydrogen bond in enzyme molecule，and change the enzyme molecule structure，consequently make enzyme be prone to lose its activity.

How to solve the above-mentioned problems？People have been searching all kinds of solutions all the time, such as choosing high temperature enduring enzyme strain，adopting some preparation techniques such as micro-cyst or embed in order to increase the heat endurance of enzyme, and even transplanting enzyme gene to salivary gland of pigs.

Adopting liquid enzyme spraying to cool pelleted feed can completely avoid the adverse influence of temperature, pressure, shearing force and moisture in feed manufacturing processes such as pelleting and expanding, which is an effective solution to the problem.

The Character of Microtech 5000L

Product standard：Enzyme activity=5000U/g

The definition of phytase activity unit：under the condition of 37℃，pH  5.0，the amount of phytase needed to hydrolyze phytate sodium zymolyte and produce 1μmol of inorganic phosphorus in 1 minute is one phytase activity unit.

Physical character：Brown to dark brown liquid，can be mixed with water in any proportion.

pH: 5.0-6.0.

Density: 1.05-1.2 g/ml.

Viscosity: 3-8 mPaS.

The Advantage of Microtech 5000L Product

1. High effect gene project strain Pichia Pastoris express，high enzyme activity,  worthy quality cost ratio，high security.

2. Double peaks phytase，wider effective pH value range，can make longer effect in longer section of alimentary tract（craw  stomach - forepart of small intestine）.

3. With stabilization treatment in liquid form，the product has good storage stability. The disadvantages of usual liquid enzyme such as instability and shorter storage period have been overcome.

4. Liquid form of preparation，adding through spraying after pelleting.

n    Adopting the special equipment for spraying after pelleting，completely avoid any destroy of enzyme activity caused by disadvantage factors in feed making process such as high temperature. Not necessary for any excess adding，consequently lower the cost.

n    Having avoided the high temperature in processing，the additive dosage can strictly conform to the formula，assure exact and reliable execution of the formula..

n    Needn't to carry out the premixing procedure step by step， therefore reduce some  manufacturing process，and decrease the error rate of manual operation.

n   Imported advanced spraying equipment with precise computation assures even spraying, and lower down the powder rate in the feed.

5. Strong resistance to protein enzyme and stomach acid，insensitive to various depressors. Can work stably and effectively in animal's alimentary tract.

Applying Object

Pellet feed for pig, chicken, duck and other monogastric livestock and poultry.

Suggested Dosage

Using Method

The phosphor substituting rate should be adjusted according to different animal kinds and different feed material compositions，at the same time use stone powder to complement the calcium amount of substituted  phosphor source. And the surplus space can be used to adjust formula or to be complemented by some cheaper materials such as zeolite powder.

Use the liquid spraying equipment to spray the product to the pellet feed after pelleting，detailed using method please refer to the operation manual of the spraying equipment.

Storage and Shelf life

l   Shelf life for storing in cold room (2-8℃) is 24 months.

l  Shelf life for storing in room temperature (under 25℃) is 6 months.

l  Suggest storage temperature no higher than 25℃.

Precautions

n   The additive dosage of the product is rather small, please make sure to mix evenly in adding.

n   Avoid inhaling excessive amount of the product to human aspiratory tract in using.

n   Keep away from any poisonous or noxious substance in transportation and storage.

Packing

By tank, 25 Kg/Tank

Production License No.: Feed Additive (2005) 0751

Executing Standard: Q/YDL112-2006