Composition

YIKANGSU-Ⅱ are made by several well-chosen aquatic immunity polysaccharide and oligosaccharide substance according to scientific proportion, which contains microbe culture material for special aquatic immunity factor, strong pertinence to aquatic animals!

Sick mechanism of Aquatic animals

At present, aquatic breed is highly concentrated, high density, serious water pollution. Quantity of Pathogenic disease increases largely. Immunity ability of aquatic animals is very low, especially shrimp, crab and other invertebrate, once environment get worse or polluted, quantity of pathogenic disease increases, temperature, PH value, Oxygen in solution, Nitrogen and ammonia in solution and other water quality factor change greatly, corporeity of aquatic animals will be weaken, which will create chance for nosogenetic microbe intrude body and lead infection, and cause sick.

Non-specific immunity system of aquatic animals

Non-specific immunity system is the main line of defence for infection of invertebrate, as well as defence for microbe invasion, which are large quantity of cell, anti-bacteria protein, glycoprotein, ? etc in the organism tissue and body fluid. Immunity cell includes plaque cell (plaque neutral cell and macrophage cell) and NK cell, these cells and lymphocytes in the immunity system affect each other through cell factor; anti-bacterial protein includes mucilage glue insulin, metal ion chelated preparation, proteinase depressive factor, bacteriolysin, alexin and exogenous agglutinin; interferon system is the vital part of non-specific defence mechanism. Aquatic animals have no mature immunity system, mainly depend on non-specific immunity system to defense infection. Shrimp and crab as low invertebrate rely much more on non-specific immunity system. So, how to enhance the non-specific immunity system of aquatic animals is paid more attention by people!

The functional mechanism of YI KANGSU-Ⅱ

fish and shrimp are always stimulated in concentrated breed environment, its resistance to virus decreases constantly because of depress, once it is attacked by pathogeny, sick can easily happen. In order to cure disease, people always use all kinds of anti-bacterial medicine, the medicine dosage becomes more and more large because of medicine resistance, which create malignant recycle, medicine resistance bacteria and medicine residue etc problem. These problem directly

harm human being's health. As a type of natural feed additive, YIKANGSU-Ⅱ mainly focus on improving aquatic animals defense ability of itself, enhancing non-specific immunity in a short time, which can increase resistant ability to virus and bacteria, thereby keep health.

The functional mechanism of polysaccharide

î        Ⅰ.Immunity polysaccharide is the given sequence structure reserved by microbe evolution proceed, body fluid factor and phagocyte receiver in invertebrate and vertebrate all can recognize polysaccharide on the cell wall of these microbe, so it can stimulate non-specific immunity system as immunity stimulated preparation.

î        II.Improve production of lysozyme in animal blood serum, improve activity of alexin, enhance non-specific immunity function.

î        III.Stimulate il-2 created by macrophage, IL-2 can place a premium on creating IFNγor other  similar substance MAF (macrophage activity factor) in NK cell or T lymph cell, increase phagocyte ability and stimulate macrophage to create TNF-αfactor, which has anti-bacteria and anti-virus ability.

The functional mechanism of immunity oligosaccharide

î        Induce the mRNA express of all kinds of immunity activity substance, such as interleukin, interferon and so on; enhance macrophage activity and serial immunity system of animals.

î        II. Secrete a type of special protein by stimulating liver, which can touch off a series of alexin through combining with bacteria ?, therefore improve non-specific immunity and adjust body fluid and bacteria immunity function, form system of defencing disease.

The main function:

î        ---Enhance immunity function, improve resistant abilty to bacteria, virus, protist and other pathogenic microbe.

î        ---The aquaculture's livers get protect than before, enhance liver's detoxification and nutrition transform ability.

î        ---Stimulate cell division and protein synthesis of aquaculture, thereby promote growth and weight gain.

î        ---Stimulate chemical receiver of aquatic animal, induce in take, improve feed utilization rate.

î        ---Proliferate anaerobic beneficiary bacteria in water, reform water quality and level off PH value.

Application result

Many comparisom experiments of Yikangsu-II have been done in different feed formulation in china, The representative experiment result showes as following:

Immunity mechanism and anti-disease ability

Comparing with control group ,the blood cell quantity is increased by 165.21% (p<0.01) and 143.13%(p<0.01) respectively; The phagocyte Swallowing index is increased by 45.35%(0.05) and 11.63%(0.05) respectively; the activity of lymphocyte phenolase is increased by 44.17%(p<0.01) and 38.66%(p<0.01)respectively; The agglutination titre to blood serum is increased by 42.86% and 242.86%(p<0.01) respectively; anti-bacteria effects of staphylococcus aureus is increased by 4.83% and 21.14% respectively in treatment group 1 and 2.

(Study report of Hainan University of china)

Survival rate of prawn after WSSV infection (%)

Infected by WSSV after 60 days, the control group has no survivor, survival rate of treatment group 1 and 2 are 15% and 20% respectively.

(Study report of Hainan University)

survival rate of eel after infection by ?

Comparing with control group, survival rate are increased from 17% to 58% and 70% respectively in treatment group 1 and 2.

Performance

Difference addition of Yikangsu-II on reforming of

body weight gain and bait coefficient

Added 0.05% to 0.02% yikiangsu could increase body weight by 10.31% (p<0.05)、17.81% （p<0.01）、17.91% (p<0.01) and 12.19% (p<0.01)  respectively; reduced bait coefficient by 2.21%、4.41%、3.68% and 2.94% respectively.

The effect of Yikangsu-II on reforming of grass carp's body weight gain and bait coefficient

After feeding Yikiangsu-Ⅱ, increase body weight gain, reduce bait coefficient. If yikiangsu-Ⅱand

yiduozyme898A were used together, which get better effects.

      Compare with control group production improving rate per MU(1 MU=667sqm) %(aquatic farm A)

      Compare with control group production improving rate per MU(1 MU=667sqm) %(aquatic farm B)

      Compare with control group, average production improving rate per MU %.

Effect of different treatment of yikiangsu-Ⅱ on production improving rate per MU (aquatic farm A 、B and average value)

Compare with control group, the production improving rate per MU%.

    Compare with control group average weight gain rate%(aquatic farm A)

    Compare with control group average weight gain rate%(aquatic farm B)

    Compare with control group average weight gain rate%(farm A&B).

The effect of difference treatment of yikiangsu-Ⅱ on improving rate of average weight gain.(Farm A and B, average value)

Compare with control group, body weight gain improving rate%.

The effects of different treatment yikiangsu-Ⅱ on survival rato.

Compare with control group, the survival rate increased rate%

Practical application effect of Yikangsu-II in 2 farms in Hainan:

Treatment 1: feed 0.20% yikiangsu-Ⅱonce per day

Treatment 2: feed 0.2% yikiangsu-Ⅱtwice per day

Treatment3: feed 0.2% yikiangsu-Ⅱand 0.1% yiduozyme898A once per day                Treatment 4: control group

Result showed that different treatment yikiangsu-Ⅱ could increase production per MU, weight gain, survival rate; average showed that if dosage of yikiangsu-Ⅱ got increased ,the body weight got better , moreover yikiangsu-Ⅱ can cooperate with Yiduozyme to have effect on weight gain.

(The study report of Hainan University)

Treatment 1--0.05% Yikangsu-II

Treatment 2--0.10% Yikangsu-II

Treatment 3--0.15% Yikangsu-II

Treatment 4--0.20% Yikangsu-II

The effect of yikiangsu-Ⅱon body composition of shrimp

With dosage of yikiangsu-Ⅱ was increased, the content of protein in shrimp composition gets increased .The carbohydrate gets reduced.

(The study report of Zhanjiang Ocean University)

Other applications

Most customers in different place of china always used yikiangsu-Ⅱ as “douse preparation”. The method is to douse 1kg of yikiangsu-Ⅱ per MU water on the 2M deep sea, Which could stabilize water quality, such as PH value; After 23 days, the shell of shrimp became (body colour) light, bright, smooth, and good-looking, original neuroscience shell gets better effects; the feed in take

increased obviously. Once douse can last more than 1 week.

Application objects:

Shrimp, fish, frog, crab and turtle and other aquatic animals.

Usage:

î        Directly apply in all kinds of aquatic feed (includes expanded feed)

î        Directly add and mix in feed

î        Cooperation with yiduozyme brings better effects.

î        Directly douse in as water quality improvement preparation

Recommended usage:

For shrimp, crab :0. 10%-0. 20%           For Fish:0. 05%-0. 10%

Water quality improvement preparation: 1kg/MU/week

Characters:

î        No formulation tabu

î        Endure high temperature and other extreme-processing conditions

î        Natural, No residual

Precautions:

Avoid moisture; if there is remainder after using, the inner and outer packages should be tightened.It doesn't affect quality if product absorbs moisture.

Package and storage:

---Color compound aluminum-foil package,

1kg/bag and 0.5kg/bag.

---Keep in dry, keep away from toxic substances and pollution.

Shelf period: 12 months

Technique service:

VTR company has set up more than 20 branches in china, our experts who are engaged in animal nutrition industrial for long term will provide high-quality technical service and updated information for you in the whle country.